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GrainGenes Marker Report: Xcfd168-2D.1

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Locus	Xcfd168-2D.1
Type	Microsatellite
Map	Ta-Synthetic/Opata-GPW-2D 155.9
	[ Show Nearby Loci ]
Map Data	Wheat, Synthetic x Opata, GPW
Probe	CFD168
Possible Orthologs	Xcfd168 [ Show all 8 ]
Probe for Locus	CFD168
Locus	[ Hide all but 1 of 9 ] Xcfd168-2D.1 Xcfd168-2D.2 Xcfd168-2D Xcfd168 Xcfd168b Xcfd168-2A cfd168 cfd168-2A cfd168-2D
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CTG)20 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' CTTCGCAAATCGAGGATGAT 3' Reverse 5' TTCACGCCCAGTATTAAGGC 3'
SSR size	197
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	2D
Source Species	Aegilops tauschii