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GrainGenes Marker Report: Xcfd13a

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Locus	Xcfd13a
Type	Microsatellite
Chromosome	6B 6D
Map	Wheat-Composite2004-6B 83 Wheat-Composite2004-6D 45
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	CFD13
Possible Orthologs	[ Hide all but 1 of 14 ] Xcfd13 Xcfd13-6B Xcfd13-6B.1 Xcfd13-6D Xcfd13b cfd13 cfd13-6B cfd13_1 cfd13_2 cfd13-6D cfd13-6B.1 cfd13-6B.2 cfd13-6D.1 cfd13-6D.2
Probe for Locus	CFD13
Locus	Xcfd13-6B [ Show all 16 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CT)20(TGTA)3 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' CCACTAACCAAGCTGCCATT 3' Reverse 5' TTTTTGGCATTGATCTGCTG 3'
SSR size	254
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	6B/6D
Source Species	Aegilops tauschii