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GrainGenes Marker Report: Xcfd11.2

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Locus
Xcfd11.2
Type
Microsatellite
Map
Ta-NxW-2B102.7
[ Show Nearby Loci ]
Map Data
Wheat, Nanda2419 x Wangshuibai
Probe
CFD11
Possible Orthologs
Xcfd11-2B
Xcfd11-2D
Xcfd11.1
Xcfd11a
Xcfd11b



Probe for Locus
CFD11
Locus
[ Hide all but 1 of 6 ]
Xcfd11-2D
Xcfd11-2B
Xcfd11.2
Xcfd11.1
Xcfd11b
Xcfd11a
Reference
ReferenceGuyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = B
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (CA)31
Regularity of motif is imperfect
Type
PCR
SSR
PCR primers
Forward 5' GGATTTGCCTTGGATTTGAA 3'
Reverse 5' TTGCATCTCACGCACCTAAG 3'
SSR size
167
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
2D/2B
Source Species
Aegilops tauschii