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GrainGenes Marker Report: Xcfd102.1-5D

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Locus	Xcfd102.1-5D
Type	Microsatellite
Map	P91193D1/P92201D5-5D 56.8
	[ Show Nearby Loci ]
Map Data	Wheat, P91193D1 x P92201D5
Probe	CFD102
Possible Orthologs	Xcfd102-5D Xcfd102.2-5D cfd102-5D cfd102 Xcfd102
Probe for Locus	CFD102
Locus	[ Hide all but 1 of 6 ] Xcfd102-5D Xcfd102.2-5D Xcfd102.1-5D cfd102-5D cfd102 Xcfd102
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = A Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CT)12(CA)16(CT)27 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TTGTGGAAGGGTTTGATGAAG 3' Reverse 5' TGCAGGACCAAACATAGCTG 3'
SSR size	299
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	5D
Source Species	Aegilops tauschii