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GrainGenes Marker Report: Xcfd10-2D

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Locus	Xcfd10-2D
Type	Microsatellite
Map Data	Wheat, Consensus SSR, 2004
Probe	CFD10
Possible Orthologs	Xcfd10 Xcfd10-3D Xcfd10-5D cfd10-5D cfd10
Probe for Locus	CFD10
Locus	[ Hide all but 1 of 6 ] Xcfd10-5D Xcfd10 cfd10-5D cfd10 Xcfd10-2D Xcfd10-3D
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)16 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' CGTTCTATGACGTGTCATGCT 3' Reverse 5' TCCATTTTCAAAAACACCCTG 3'
SSR size	288
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	5D
Source Species	Aegilops tauschii