GrainGenes Marker Report: Xcfa2170-3A.2
Locus
Xcfa2170-3A.2
Type
Microsatellite
Chromosome
3A
Chromosome Arm
3AL
Map
Triticum-AxF-3A 145.6
[ Show Nearby Loci ]
Map Data
Wheat, Arina x Forno
Species
Triticum aestivum
Probe
CFA2170
Reference
Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
Possible Orthologs
cfa2170
[ Show all 8 ]
Probe for Locus
CFA2170
Locus
[ Hide all but 1 of 10 ] Xcfa2170-3B Xcfa2170-1D Xcfa2170-3A Xcfa2170a Xcfa2170-3A.1 Xcfa2170-3A.2 Sr35 cfa2170 cfa2170-3A cfa2170-3B
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)31 Regularity of motif is imperfect
Type
PCR SSR
PCR primers
Forward 5' TGGCAAGTAACATGAACGGA 3'
Reverse 5' ATGTCATTCATGTTGCCCCT 3'
SSR size
199
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
3A/3B
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2170-3A.2
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