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GrainGenes Marker Report: Xcfa2170-3A

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Locus
Xcfa2170-3A
Type
Microsatellite
Map
P91193D1/P92201D5-3A126.9
T.b.xT.m.-3A202.4
Wheat, UC1110 x PI610750 3A143.7
Wheat_Brittle_culm_2012-3A3.7
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Map Data
T. boeoticum x monococcum
Wheat, 2012, Brittle culm
Wheat, P91193D1 x P92201D5
Wheat, UC1110 x PI610750
Probe
CFA2170
Reference
ReferenceLowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs
[ Hide all but 1 of 8 ]
cfa2170
Xcfa2170-1D
Xcfa2170-3A.1
Xcfa2170-3A.2
Xcfa2170-3B
Xcfa2170a
cfa2170-3A
cfa2170-3B



Probe for Locus
CFA2170
Locus
Xcfa2170-3B
[ Show all 10 ]
Reference
ReferenceSourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (GA)31
Regularity of motif is imperfect
Type
PCR
SSR
PCR primers
Forward 5' TGGCAAGTAACATGAACGGA 3'
Reverse 5' ATGTCATTCATGTTGCCCCT 3'
SSR size
199
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
3A/3B
Source Species
Triticum urartu