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GrainGenes Marker Report: Xcfa2153-1A

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Locus	Xcfa2153-1A
Type	Microsatellite
Chromosome	1A
Chromosome Arm	1AS
Map	[ Hide all but 1 of 6 ] Chinese_Spring_Deletion_SSR_1A -0.93 CS/SQ1-1A 6.8 P91193D1/P92201D5-1A 22.3 T.b.xT.m.-1A 55.7 Ta-Courtot/CS-GPW-1A 5.4 Triticum-AxF-1A 1
	[ Show Nearby Loci ]
QTL with Locus Marker	QCre.pau-1A
Breakpoint Interval	1AS3-0.86-1.00
Map Data	T. boeoticum x monococcum [ Show all 6 ]
Species	Triticum aestivum
Probe	CFA2153
Reference	Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
Possible Orthologs	Xcfa2153 Xcfa2153-1A.1 Xcfa2153-1A.2
Probe for Locus	CFA2153
Locus	Xcfa2153-1A Xcfa2153-1A.1 Xcfa2153-1A.2 Xcfa2153 H9/H10/H11
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = A Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CT)27 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TTGTGCATGATGGCTTCAAT 3' Reverse 5' CCAATCCTAATGATCCGCTG 3'
SSR size	200
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1A
Source Species	Triticum urartu