GrainGenes Marker Report: Xcfa2129a
Locus
Xcfa2129a
Type
Microsatellite
Map
Svevo/Ciccio-1B 126.2
[ Show Nearby Loci ]
Breakpoint Interval
1BL2-0.69-0.85
Map Data
Durum wheat, Svevo x Ciccio
Probe
CFA2129
Possible Orthologs
Xcfa2129
[ Show all 14 ]
Probe for Locus
CFA2129
Locus
[ Hide all but 1 of 15 ] Xcfa2129-1A Xcfa2129-1B Xcfa2129-1D Xcfa2129a Xcfa2129 cfa2129 cfa2129-1A cfa2129a cfa2129a-1B cfa2129b-1B Xcfa2129a-1B Xcfa2129b Xcfa2129b-1A cfa2129-1B.1 cfa2129-1D
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)25 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' GTTGCACGACCTACAAAGCA 3'
Reverse 5' ATCGCTCACTCACTATCGGG 3'
SSR size
159
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
1A
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2129a
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