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GrainGenes Marker Report: Xcfa2040-7B

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Locus
Xcfa2040-7B
Type
Microsatellite
Chromosome
7B
Map
Kofa/UC1113-7B183.4
Ta-SSR-2004-7B143.4
Ta-Synthetic/Opata-GPW-7B182.6
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113
Wheat, Consensus SSR, 2004
Wheat, Synthetic x Opata, GPW
Probe
CFA2040
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfa2040
[ Show all 23 ]



Probe for Locus
CFA2040
Locus
[ Hide all but 1 of 25 ]
Xcfa2040
Xcfa2040-7A
Xcfa2040-7D
Xcfa2040-1D
Xcfa2040-2D
Xcfa2040-7B
Xcfa2040b
Sr43
cfa2040
cfa2040-7A
cfa2040a
cfa2040a-7B
cfa2040b
cfa2040b-7B
cfa2040_1
cfa2040_2
cfa2040_3
cfa2040-7A.1
cfa2040-7A.2
cfa2040-7D.1
cfa2040-7D.2
cfa2040-7B.1
cfa2040-7B.2
cfa2040-7B.3
cfa2040-7B
Reference
ReferenceSourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (CA)16
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' TCAAATGATTTCAGGTAACCACTA 3'
Reverse 5' TTCCTGATCCCACCAAACAT 3'
SSR size
286
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
7A/7D
Source Species
Triticum urartu