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GrainGenes Marker Report: WMC479

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Probe	WMC479
Locus	[ Hide all but 1 of 10 ] Xwmc479-7A Xwmc479 wmc479a wmc479a-7A wmc479b wmc479b-7A wmc479 wmc479-7A.1 wmc479-7A.2 wmc479-7A.3
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25e4.2 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 199 bases located on 7A Multiple inserts in this clone, this is the second insert. Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)16 259 to 290.
Type	PCR SSR
PCR primers	Forward gAccTAAgcccAgTgTcATcAg at 162 length 22 bases, Tm 63 Reverse AgAcTcTTggcTTTggATAcgg at 336 length 22 bases, Tm 63
SSR size	196 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC479
Source Species	Triticum aestivum