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GrainGenes Marker Report: Suk9M49c

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Locus	Suk9M49c
Type	SSAP
Chromosome	2H
Map	Hordeum-SxM-SSAP-2H 61.9
	[ Show Nearby Loci ]
Map Data	Barley, Steptoe x Morex, SSAP
Probe	Suk9M49
Reference	Rodriguez M et al. (2006) Integration of retrotransposons-based markers in a linkage map of barley. Molecular Breeding 17:173-184.
Probe for Locus	Suk9M49
Locus	[ Hide all but 1 of 13 ] Suk9M49b Suk9M49h Suk9M49c Suk9M49j Suk9M49k Suk9M49d Suk9M49i Suk9M49m Suk9M49l Suk9M49g Suk9M49a Suk9M49e Suk9M49f
Reference	Leigh F et al. (2003) Comparison of the utility of barley retrotransposon families for genetic analysis by molecular marker techniques Molecular Genetics and Genomics 269:464-474.
General Remarks	Position of retroposon-based primer in the LTR = 3' and 1 nucleotide internal to the LTR terminus MseI primer (KeyGene code) = CAG (M49)
Type	PCR STS
PCR primers	Sukkula-9900 5' GATAGGGTCGCATCTTGGGCGTGAC 3' Mse1 primer M49 5' GATGAGTCCTGAGTAACAG 3'
Amplification Conditions	amplified as Vos et al Nucl Acids Res 23: 4407-4417
Source Species	Hordeum vulgare