GrainGenes Marker Report: Sr33
Locus
Sr33
Type
Gene Trait
Chromosome
1D
Chromosome Arm
1DS 1S
Map
1DS, Ae. tauschii 30.7
[ Show all 6 ]
[ Show Nearby Loci ]
Map Data
1DS, Ae. tauschii Triticeae consensus Wheat genes Wheat, Synthetic x Opata [ GBrowser ] Wheat-2014-Consensus-Ug99-Update
2 Point Data
Centromere/Sr33#1 Rg2/Sr33#1 Distance: 35 cM Sr33/Gli-D1#1 Distance: 9 cM
Species
Aegilops tauschii Triticum aestivum
Probe
ABC156 BARC152 CFD15
Associated Gene
Sr33 (Aegilops) Sr33 (Triticum)
Gene Class
Reaction to Puccinia graminis Pers.
Reference
[ Hide all but 1 of 6 ] 
Yu L et al. (2014) A consensus map for Ug99 stem rust resistance loci in wheat. Theoretical and Applied Genetics 127:1561-1581.
Periyannan S et al. (2013) The Gene Sr33, and ortholog of Barle MIa Genes, Encodes Resistance to Wheat Stem Rust Race Ug99 Science 341:786-788.
Mago R et al. (2002) Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines Theoretical and Applied Genetics 104:1317-1324.
Spielmeyer W et al. (2000) NBS-LRR sequence family is associated with leaf and stripe rust resistance on the end of homoeologous chromosome group 1S of wheat Theoretical and Applied Genetics 101:1139-1144.
Hart GE et al. (1993) Linkage maps of Triticum aestivum (hexaploid wheat, 2n = 42, genomes A, B, and D) and T. tauschii (2n = 14, genome D). Genetic maps: Locus Maps of Complex Genomes.
MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr33 MAS Wheat. Marker Assisted Selection in Wheat.
Data Source
Matthews, David E.
Remark
For map 'Wheat, Synthetic x Opata', position approximated from mapping in another population. Trait marker abc156, whs179a Nearby marker on Composite map: Xabc156b, Xwhs179.1 Position on Composite map estimated by GrainGenes. The location on the 2014 Ug99 Consensus Map is approximate.
Probe for Locus
CFD15
Locus
Xcfd15-1A
[ Show all 11 ]
Reference
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CT)20(TGTA)3 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' CTCCCGTATTGAGCAGGAAG 3'
Reverse 5' GGCAGGTGTGGTGATGATCT 3'
SSR size
179
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
1A/1D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Sr33
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