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GrainGenes Marker Report: NikM62h

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Locus	NikM62h
Type	SSAP
Chromosome	7H
Map	Hordeum-SxM-SSAP-7H 77.9
	[ Show Nearby Loci ]
Map Data	Barley, Steptoe x Morex, SSAP
Probe	NikM62
Reference	Rodriguez M et al. (2006) Integration of retrotransposons-based markers in a linkage map of barley. Molecular Breeding 17:173-184.
Probe for Locus	NikM62
Locus	[ Hide all but 1 of 11 ] NikM62h NikM62a NikM62e NikM62k NikM62g NikM62f NikM62c NikM62j NikM62d NikM62i NikM62b
Reference	Leigh F et al. (2003) Comparison of the utility of barley retrotransposon families for genetic analysis by molecular marker techniques Molecular Genetics and Genomics 269:464-474.
General Remarks	Position of retroposon-based primer in the LTR = 5' and 80 nucleotides internal to the LTR terminus MseI primer (KeyGene code) = CTT (M62)
Type	PCR STS
PCR primers	Nikita-E2647 5' ACCCCTCTAGGCGACATCC 3' Mse1 primer M62 5' GATGAGTCCTGAGTAACTT 3'
Amplification Conditions	amplified as Vos et al Nucl Acids Res 23: 4407-4414
Source Species	Hordeum vulgare