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GrainGenes Marker Report: MWG2200

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Locus	MWG2200
Type	CAPS RFLP
Chromosome	2H
Map	[ Hide all but 1 of 7 ] Barley-ConsensusFHB-2H 154.41 Hordeum-Bins-2H 167 Hordeum-Consensus2007-SSR-2H 154.42 Hordeum-Graner2-2H 191 Hv-Consensus2006-Marcel-2H 154.42 Hv-Integrated2009-2H 150.9 Hv-L94/Vada-2006-2H 191.67
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Map Data	Barley, BinMap 2005 [ Show all 7 ]
Species	Hordeum vulgare
Probe	MWG2200
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Data Source	Kleinhofs, Andris
Remark	This marker is a Binlocus in the Hordeum-Bins maps, i.e. a marker mapped in other crosses and located to the Steptoe x Morex bin Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = L9Oregon Wolfe Barleys x Vada,Igri x Franka Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Igri x Franka Locus in 'Barley, Consensus 2007, SSR' was a BIN marker in 'Barley, Consensus 2006, Marcel'
Probe	MWG2200
Locus	MWG2200
Reference	Marcel TC et al. (2007) A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues. Theoretical and Applied Genetics 114:487-500.
Remarks	Restriction Enzyme for CAPS marker is Ava I
PCR primers	5' agcacggcctccagcaccac 3' 5' agcacggcctccagcaccac 3' 5' atcctggagtacctcgaagccg 3' 5' atcctggagtacctcgaagccg 3'
STS primers	5' ATCCTGGAGTACCTCGAAGCCG 5' CGATGGACGTGCATCTAAGTCG
STS size	272
Amplification Conditions	5 min at 94 deg C; 35 cycles with 30 sec at 94 deg C, 30 sec at 58 deg C, and 1 min at 72 deg C; and a final extension step of 7 min at 72 deg C
Sequence	AJ234832 MWG2200-5
Source Species	Hordeum vulgare