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GrainGenes Marker Report: HVPLASC1B

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Locus	HVPLASC1B
Type	Microsatellite
Chromosome	7H
Map	Barley-ConsensusFHB-7H 7.16 Hordeum-Consensus2006-DArT-7H 0 Hordeum-Consensus2007-SSR-7H 2.65 Hordeum-LxHs-7H 8 Hv-Integrated2009-7H 6.8
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, DArT Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009 Barley, LxHs
Species	Hordeum spontaneum Hordeum vulgare
Probe	HVPLASC1B
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	[ Hide all but 1 of 7 ] Number of populations contributing to the placement of HvPLASC1B in the Barley, Consensus 2006 DArT maps = 1 Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Nure x Tremois Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (TCTAC)4 Polymorphic Information Content (PIC) = 0.32 Developing laboratory = SCRI (R. Waugh)
Probe	HVPLASC1B
Locus	HVPLASC1B
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (TCTAC)4 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.32
Type	PCR SSR
PCR primers	5' GTGCATGCATCATATTGATTA 3' 5' ACGTACGTACTTATCACGAAGA 3' Forward GTGCATGCATCATATTGATTA Reverse ACGTACGTACTTATCACGAAGA GTGCATGCATCATATTGATTA ACGTACGTACTTATCACGAAGA
SSR size	110
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare