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GrainGenes Marker Report: GBM1036

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Locus	GBM1036
Type	Microsatellite
Chromosome	2H
Map	Barley, B73xCPI-2H 176.7 Barley-ConsensusFHB-2H 156.38 Hordeum-Consensus2007-SSR-2H 156.39 Hv-Consensus2006-Marcel-2H 156.39 Hv-Integrated2009-2H 152.6
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Map Data	Barley, B73xCPI Barley, Consensus 2006, Marcel Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009
Species	Hordeum vulgare
Probe	GBM1036
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Thiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remark	[ Hide all but 1 of 7 ] Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Steptoe x Morex,Oregon Wolfe Barleys Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Steptoe x Morex; Oregon Wolfe Dom x Rec Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Steptoe x Morex, OWBrecessive x OWBdominant Insert contains repeat (CT)8 Polymorphic Information Content (PIC) = 0.61 Developing laboratory = IPK (A. Graner)
Probe	GBM1036
Locus	GBM1036 GBM1036-2H
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Thiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remarks	Primer sequences for this SSR are proprietary, those interested should contact Andreas Graner Clone HW04F09u
Type	SSR EST
PCR primers	available upon request to authors: graner@ipk-gatersleben.de
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	AL501480
DNA Library	IPK-HW
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)