Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: EBmac521

[Submit comment/correction]

Locus	EBmac521
Type	Microsatellite
Chromosome	2H
Map	Hordeum-Zhedar2-FHB-QTL-2H 102.8
	[ Show Nearby Loci ]
QTL with Locus Marker	QHD.dah-2H.1
Map Data	Barley, Zhedar2, FHB QTL
Probe	EBmac521
Possible Orthologs	[ Hide all but 1 of 6 ] Bmac0521 EBmac0521 EBmac0521.2H EBmac0521.7H EBmac521a EBmac521b
Probe	EBmac521
Locus	EBmac0521 EBmac521a EBmac521b EBmac521
Synonym	Other name EBmac0521
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)18 Quality = 2: Single product, relatively strong stutter bands
Type	PCR SSR
PCR primers	5' TGAAGCACAAGTGTGGAA 3' 5' AGACGTATCATTGCCCAC 3'
SSR size	163
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare