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GrainGenes Marker Report: EBmac0970

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Locus	EBmac0970
Type	Microsatellite
Synonym	Synonym EBmac970
Chromosome	5H
Map	[ Hide all but 1 of 8 ] Barley-ConsensusFHB-5H 55.51 Hordeum-Consensus2005-SNP-5H 43.792 Hordeum-Consensus2007-SSR-5H 40.77 Hordeum-FxS-FHB-QTL-5H 76.6 Hordeum-LiHs-SNP-5H 26.8 Hordeum-LxHs-5H 54 Hordeum-QTLConsensus-Abiotic-5H 43.79 Hordeum-QTLConsensus-Agronomic-5H 43.79
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 8 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac0970 EBmac970
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Mesfin A et al. (2003) Quantitative trait loci for fusarium head blight resistance in barley detected in a two-rowed by six-rowed population Crop Science 43:307-318. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (AC)8 Polymorphic Information Content (PIC) = 0.49 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	Bmac0970 [ Show all 6 ]
Probe for Locus	EBmac970
Locus	EBmac0970 EBmac970 EBmac970a EBmac970b
Synonym	Other name Bmac970 EBmac0970
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)8 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.49
Type	PCR SSR
PCR primers	5' ACATGTGATACCAAGGCAC 3' 5' TGCATAGATGATGTGCTTG 3'
SSR size	112
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare