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GrainGenes Marker Report: EBmac0906

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Locus	EBmac0906
Type	Microsatellite
Synonym	Synonym EBmac906
Chromosome	4H
Map	Barley, B73xCPI-4H 57 [ Show all 11 ]
	[ Show Nearby Loci ]
Map Data	[ Hide all but 1 of 11 ] Barley, Abiotic QTL Consensus Barley, Agronomic QTL Consensus Barley, B73xCPI Barley, Consensus 2005, SNP Barley, Consensus 2006, Marcel Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009 Barley, Lina x H.spont, SNP Barley, Lx41 Barley, LxHs
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac0906 EBmac906
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 7 ]
Possible Orthologs	EBmac906
Probe for Locus	EBmac906
Locus	EBmac0906 EBmac906
Synonym	Other name EBmac0906
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (GC)5GGG(GT)16 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.75
Type	PCR SSR
PCR primers	5' CAAATCAATCAAGAGGCC 3' 5' TTTGAAGTGAGACATTTCCA 3' CAAATCAATCAAGAGGCC TTTGAAGTGAGACATTTCCA
SSR size	153
Amplification Conditions	1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare