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GrainGenes Marker Report: EBmac0906

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Locus	EBmac0906
Type	Microsatellite
Synonym	Synonym EBmac906
Chromosome	4H
Map	[ Hide all but 1 of 11 ] Barley, B73xCPI-4H 57 Barley-ConsensusFHB-4H 57.42 Hordeum-Consensus2005-SNP-4H 42.011 Hordeum-Consensus2007-SSR-4H 54.98 Hordeum-LiHs-SNP-4H 55.18 Hordeum-Lx41-4H 54.5 Hordeum-LxHs-4H 37 Hordeum-QTLConsensus-Abiotic-4H 42.01 Hordeum-QTLConsensus-Agronomic-4H 42.01 Hv-Consensus2006-Marcel-4H 54.98 Hv-Integrated2009-4H 66.2
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 11 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac0906 EBmac906
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 7 ]
Possible Orthologs	EBmac906
Probe for Locus	EBmac906
Locus	EBmac0906 EBmac906
Synonym	Other name EBmac0906
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (GC)5GGG(GT)16 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.75
Type	PCR SSR
PCR primers	5' CAAATCAATCAAGAGGCC 3' 5' TTTGAAGTGAGACATTTCCA 3' CAAATCAATCAAGAGGCC TTTGAAGTGAGACATTTCCA
SSR size	153
Amplification Conditions	1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare