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GrainGenes Marker Report: EBmac0816

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Locus	EBmac0816
Type	Microsatellite
Synonym	Synonym EBmac816
Chromosome	1H
Map	[ Hide all but 1 of 8 ] Barley, B73xCPI-1H 52.3 Barley-ConsensusFHB-1H 60.2 Hordeum-Consensus2005-SNP-1H 60.162 Hordeum-Consensus2007-SSR-1H 64.13 Hordeum-LiHs-SNP-1H 51.08 Hordeum-LxHs-1H 51 Hordeum-QTLConsensus-Abiotic-1H 60.16 Hordeum-QTLConsensus-Agronomic-1H 60.16
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 8 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac0816 EBmac816
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (CA)7,(CA)7 Polymorphic Information Content (PIC) = 0.13 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	EBmac816
Probe for Locus	EBmac816
Locus	EBmac0816 EBmac816
Synonym	Other name EBmac0816
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (CA)7,(CA)7 Quality = 4: Ladders of bands that are at equal intensity, but may contain scoreable weak products Diversity Index (DI) = 0.13
Type	PCR SSR
PCR primers	5' ATTACATGGGGAGATGTCTT 3' 5' GCACATCTACACTTGTGCA 3'
SSR size	253
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare