GrainGenes Marker Report: EBmac0806
Locus
EBmac0806
Type
Microsatellite
Synonym
Synonym EBmac806
Chromosome
6H
Map
Barley, B73xCPI-6H 54.6
[ Show all 11 ]
[ Show Nearby Loci ]
Map Data
Barley, Abiotic QTL Consensus
[ Show all 11 ]
Species
Hordeum spontaneum Hordeum vulgare
Probe
EBmac0806 EBmac806
Reference
Remark
[ Hide all but 1 of 7 ] Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = L9Oregon Wolfe Barleys x Vada,Steptoe x Morex Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Steptoe x Morex Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : L94 x Vada, Steptoe x Morex, Lina x H. spontaneum Insert contains repeat (CA)4GA(CA)8, (CA)5 Polymorphic Information Content (PIC) = 0.78 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs
EBmac806
Probe for Locus
EBmac806
Locus
EBmac0806 EBmac806
Synonym
Other name Bmac806 EBmac0806
Reference
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (CA)4GA(CA)8,(CA)5 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.78
Type
PCR SSR
PCR primers
5' ACTAAGTCCTTTCACGAGGA 3'
5' GTGTGTAGTAGGTGGGTACTTG 3'
SSR size
168
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare
GrainGenes Marker Report: EBmac0806
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