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GrainGenes Marker Report: EBmac0806

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Locus
EBmac0806
Type
Microsatellite
Synonym
SynonymEBmac806
Chromosome
6H
Map
Barley, B73xCPI-6H54.6
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Map Data
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Barley, Abiotic QTL Consensus
Barley, Agronomic QTL Consensus
Barley, B73xCPI
Barley, Consensus 2005, SNP
Barley, Consensus 2006, Marcel
Barley, Consensus 2007, SSR
Barley, Consensus 2023, FHB
Barley, Integrated, Marcel 2009
Barley, L94 x Vada, 2006
Barley, Lina x H.spont, SNP
Barley, LxHs
Species
Hordeum spontaneum
Hordeum vulgare
Probe
EBmac0806
EBmac806
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
[ Show all 7 ]
Possible Orthologs
EBmac806



Probe for Locus
EBmac806
Locus
EBmac0806
EBmac806
Synonym
Other nameBmac806
EBmac0806
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (CA)4GA(CA)8,(CA)5
Quality = 1: Single product, only weak stutter bands
Diversity Index (DI) = 0.78
Type
PCR
SSR
PCR primers
5' ACTAAGTCCTTTCACGAGGA 3'
5' GTGTGTAGTAGGTGGGTACTTG 3'
SSR size
168
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare