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GrainGenes Marker Report: EBmac0775

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Locus	EBmac0775
Type	Microsatellite
Synonym	Synonym EBmac775
Chromosome	4H
Map	[ Hide all but 1 of 8 ] Barley, B73xCPI-4H 72.8 Barley-ConsensusFHB-4H 75.68 Hordeum-Consensus2005-SNP-4H 58.329 Hordeum-Consensus2007-SSR-4H 64.37 Hordeum-LiHs-SNP-4H 40.22 Hordeum-LxHs-4H 42 Hordeum-QTLConsensus-Abiotic-4H 58.33 Hordeum-QTLConsensus-Agronomic-4H 58.33
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 8 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac0775 EBmac775
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (TG)4TT(TG)17 (AG)10 Polymorphic Information Content (PIC) = 0.78 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	EBmac775
Probe for Locus	EBmac775
Locus	EBmac0775
Synonym	Other name EBmac0775
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (TG)4TT(TG)17(AG)10 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.78
Type	PCR SSR
PCR primers	5' GCTTCCTTCATAGACCCAT 3' 5' ATATCATGCCAATGGTGTC 3'
SSR size	149
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare