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GrainGenes Marker Report: EBmac0701

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Locus	EBmac0701
Type	Microsatellite
Synonym	Synonym EBmac701
Chromosome	4H
Map	Barley, B73xCPI-4H 101 [ Show all 17 ]
	[ Show Nearby Loci ]
QTL with Locus Marker	MQTL_Du2024.yield-4H.8
Map Data	[ Hide all but 1 of 17 ] Barley, Abiotic QTL Consensus Barley, Agronomic QTL Consensus Barley, B73xCPI Barley, Consensus 2005, SNP Barley, Consensus 2006, Marcel Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, FxS, FHB QTL Barley, Integrated, Marcel 2009 Barley, L94 x Vada, 2006 Barley, Lina x H.spont, SNP Barley, LxHs Barley, OWB Barley, OWB, 2004 Barley, OWB, 2005 Barley, OWB, OPA2008 Barley, OWB, SNP
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac0701 EBmac701
Reference	Szucs P et al. (2009) An Integrated Resource for Barley Linkage Map and Malting Quality QTL Alignment The Plant Genome 2:134-140. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Mesfin A et al. (2003) Quantitative trait loci for fusarium head blight resistance in barley detected in a two-rowed by six-rowed population Crop Science 43:307-318. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 7 ]
Possible Orthologs	Bmac0701 Bmac701 EBmac701
Probe for Locus	EBmac701
Locus	EBmac0701 EBmac701
Synonym	Other name Bmac701 EBmac0701
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)23 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.74
Type	PCR SSR
PCR primers	5' ATGATGAGAACTCTTCACCC 3' 5' TGGCACTAAAGCAAAAGAC 3'
SSR size	149
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare