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GrainGenes Marker Report: EBmac0684

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Locus	EBmac0684
Type	Microsatellite
Synonym	Synonym EBmac684
Chromosome	2H 5H
Map	[ Hide all but 1 of 10 ] Hordeum-Consensus2005-SNP-5H 74.628 Hordeum-LiHs-SNP-5H 56.3 Hordeum-LxHs-5H 58 Hordeum-OWB-2004-2H 62.1 Hordeum-OWB-2005-2H 63.2 Hordeum-OWB-2H 61.8 Hordeum-OWB-OPA2008-2H 72.752 Hordeum-OWB-SNP-2H 69.91 Hordeum-QTLConsensus-Abiotic-5H 74.63 Hordeum-QTLConsensus-Agronomic-5H 74.63
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 10 ]
Species	Hordeum vulgare
Probe	EBmac684
Reference	Szucs P et al. (2009) An Integrated Resource for Barley Linkage Map and Malting Quality QTL Alignment The Plant Genome 2:134-140. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.
Possible Orthologs	Bmac0684 [ Show all 7 ]
Probe for Locus	EBmac684
Locus	EBmac0684 EBmac684 EBmac0684b
Synonym	Other name Bmac684 Ebmac0684 EBmac0684
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (TA)7(TG)11,(TG)11(TTTG)5 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.64
Type	PCR SSR
PCR primers	5' TTCCGTTGAGCTTTCATACAC 3' 5' ATTGAATCCCAACAGACACAA 3'
SSR size	172
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare