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GrainGenes Marker Report: EBmac0669

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Locus	EBmac0669
Type	Microsatellite
Synonym	Synonym EBmac669
Chromosome	4H
Map	[ Hide all but 1 of 7 ] Barley-ConsensusFHB-4H 69.4 Hordeum-Consensus2005-SNP-4H 52.879 Hordeum-Consensus2007-SSR-4H 58.52 Hordeum-LiHs-SNP-4H 43.33 Hordeum-LxHs-4H 48 Hordeum-QTLConsensus-Abiotic-4H 52.88 Hordeum-QTLConsensus-Agronomic-4H 52.88
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 7 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac0669 EBmac669
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (AC)8 Polymorphic Information Content (PIC) = 0.23 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	EBmac669
Probe for Locus	EBmac669
Locus	EBmac0669 EBmac669
Synonym	Other name EBmac0669
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)8 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.23
Type	PCR SSR
PCR primers	5' TCTACACTACACATGTTATCTTGC 3' 5' GTGTTGATCTTCCTCCTAGTG 3'
SSR size	168
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare