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GrainGenes Marker Report: EBmac0658

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Locus	EBmac0658
Type	Microsatellite
Synonym	Synonym EBmac658
Chromosome	4H
Map	[ Hide all but 1 of 9 ] Barley, B73xCPI-4H 81.2 Barley-ConsensusFHB-4H 85.63 Hordeum-Consensus2005-SNP-4H 68.763 Hordeum-Consensus2007-SSR-4H 75.59 Hordeum-LiHs-SNP-4H 61.09 Hordeum-LxHs-4H 62 Hordeum-Pillen-4H 62 Hordeum-QTLConsensus-Abiotic-4H 68.76 Hordeum-QTLConsensus-Agronomic-4H 68.76
	[ Show Nearby Loci ]
QTL with Locus Marker	QHea.pil-4H.3 QHei.pil-4H.4
Map Data	Barley, Abiotic QTL Consensus [ Show all 9 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac0658 EBmac658
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (GT)8 Polymorphic Information Content (PIC) = 0.24 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	EBmac658
Probe for Locus	EBmac658
Locus	EBmac0658 EBmac658
Synonym	Other name EBmac0658
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (GT)8 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.24
Type	PCR SSR
PCR primers	5' GTATGCAAGTGTAGGTGTGTG 3' 5' CATGGGTTTACCCACATAC 3'
SSR size	198
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare