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GrainGenes Marker Report: EBmac0623

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Locus	EBmac0623
Type	Microsatellite
Synonym	Synonym EBmac623
Chromosome	2H
Map	[ Hide all but 1 of 9 ] Barley, B73xCPI-5H 171 Barley-ConsensusFHB-2H 72.97 Hordeum-Consensus2005-SNP-2H 67.214 Hordeum-Consensus2007-SSR-2H 67.01 Hordeum-CxB-2H 25 Hordeum-LiHs-SNP-2H 63.14 Hordeum-LxHs-2H 50 Hordeum-QTLConsensus-Abiotic-2H 67.21 Hordeum-QTLConsensus-Agronomic-2H 67.21
	[ Show Nearby Loci ]
QTL with Locus Marker	MetaQ.FHB_2H_21 MetaQ.SA_2H_1 QSb.CaBo-2H
Map Data	Barley, Abiotic QTL Consensus [ Show all 9 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac0623 EBmac623
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (TG)11,(GA)10, (AG)7 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	EBmac623
Probe for Locus	EBmac623
Locus	EBmac0623 EBmac623
Synonym	Other name EBmac0623
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (TG)11,(GA)10,(AG)7 Quality = 1: Single product, only weak stutter bands
Type	PCR SSR
PCR primers	5' CGAACATTGTCGTGTTAGTAA 3' 5' CTGTCATGCATAACCTATGG 3'
SSR size	154
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare