GrainGenes Marker Report: EBmac0541
Locus
EBmac0541
Type
Microsatellite
Chromosome
3H
Map
Barley, B73xCPI-3H 118.2
[ Show all 7 ]
[ Show Nearby Loci ]
Map Data
[ Hide all but 1 of 7 ] Barley, B73xCPI Barley, Consensus 2006, Marcel Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, FxS, FHB QTL Barley, Integrated, Marcel 2009 Barley, LxHs
Species
Hordeum spontaneum Hordeum vulgare
Probe
EBmac0541 EBmac541
Reference
Remark
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
[ Show all 7 ]
Possible Orthologs
Bmac0541 EBmac541
Probe for Locus
EBmac541
Locus
EBmac0541 EBmac541
Synonym
Other name EBmac0541
Reference
Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AC)9 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.26
Type
PCR SSR
PCR primers
5' ACGGATCTACTTTAGCTAGCA 3'
5' AAACAACCCCACACAATC 3' ACGGATCTACTTTAGCTAGCA
AAACAACCCCACACAATC
SSR size
106
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare
GrainGenes Marker Report: EBmac0541
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