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GrainGenes Marker Report: CFD6

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Locus	cfd6
Type	microsatellite
Map	[ Hide all but 1 of 8 ] Durum Wheat, NSGC Lr 3A 83.2 Physical-3B 57.67 Wheat, Yr genes and QTL 2A 43.9 Wheat, Yr genes and QTL 3A 49.2 Wheat, Yr genes and QTL 3B 38.2 Wheat, Yr genes and QTL 7A 26.2 Wheat, Yr genes and QTL 7B 57.6 Wheat_AB_2017_Carotenoid_QTL-7B 145.5
	[ Show Nearby Loci ]
Map Data	3B Physical Durum Wheat, NSGC Lr Wheat AB, Carotenoid QTL, 2017 Wheat, Yr genes and QTL
Probe	CFD6
Possible Orthologs	Xcfd6 [ Show all 9 ]
Probe	CFD6
Locus	Xcfd6-7A [ Show all 10 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
Remarks	Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)6(GCTA)4 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' ACTCTCCCCCTCGTTGCTAT 3' Reverse 5' ATTTAAGGGAGACATCGGGC 3'
SSR size	236
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	7A
Source Species	Aegilops tauschii