Query (optional)   in Class  

GrainGenes Marker Report: CFA2170

[Submit comment/correction]

Locus
cfa2170
Type
microsatellite
Map
Durum Wheat, NSGC Lr 3A148
[ Show all 6 ]
[ Show Nearby Loci ]
Map Data
3B Physical
Durum Wheat, NSGC Lr
Wheat AB, Carotenoid QTL, 2017
Wheat, Yr genes and QTL
Probe
CFA2170
Possible Orthologs
[ Hide all but 1 of 8 ]
Xcfa2170-1D
Xcfa2170-3A
Xcfa2170-3A.1
Xcfa2170-3A.2
Xcfa2170-3B
Xcfa2170a
cfa2170-3A
cfa2170-3B



Probe
CFA2170
Locus
Xcfa2170-3B
[ Show all 10 ]
Reference
ReferenceSourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
Remarks
Quality Score = B
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (GA)31
Regularity of motif is imperfect
Type
PCR
SSR
PCR primers
Forward 5' TGGCAAGTAACATGAACGGA 3'
Reverse 5' ATGTCATTCATGTTGCCCCT 3'
SSR size
199
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
3A/3B
Source Species
Triticum urartu