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GrainGenes Marker Report: CFA2123

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Probe
CFA2123
Locus
[ Hide all but 1 of 9 ]
Xcfa2123-7A
Sr22
cfa2123
cfa2123a
cfa2123a-7A
cfa2123b
cfa2123b-7A
cfa2123-7A.1
cfa2123-7A.2
Reference
ReferenceSourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
Remarks
Quality Score = A
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (GA)30
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' CGGTCTTTGTTTGCTCTAAACC 3'
Reverse 5' ACCGGCCATCTATGATGAAG 3'
SSR size
259
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
7A
Source Species
Triticum urartu