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GrainGenes Marker Report: Bmac0187

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Locus	Bmac0187
Type	Microsatellite
Synonym	Synonym Bmac187
Chromosome	7H
Map	Barley, B73xCPI-7H 85.7 [ Show all 13 ]
	[ Show Nearby Loci ]
Map Data	[ Hide all but 1 of 13 ] Barley, Abiotic QTL Consensus Barley, Agronomic QTL Consensus Barley, B73xCPI Barley, Consensus 2005, SNP Barley, Consensus 2006, Marcel Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009 Barley, Lina x H.spont, SNP Barley, LxHs Barley, OWB Barley, OWB, 2004 Barley, OWB, SNP
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmac0187 Bmac187
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 6 ]
Possible Orthologs	Bmac187 Bmac187-7H
Probe for Locus	Bmac187
Locus	Bmac0187 Bmac187-7H Bmac187
Synonym	Other name Bmac0187
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)13(AT)9 Quality = 2: Single product, relatively strong stutter bands
Type	PCR SSR
PCR primers	5' GCTCTCTCTCAGAAAAATGAA 3' 5' GAATTATTCTAGGGCTGTGAA 3' GCTCTCTCTCAGAAAAATGAA GAATTATTCTAGGGCTGTGAA
SSR size	177
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare