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GrainGenes Marker Report: AM1a

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Locus
AM1a
Type
Microsatellite
Synonym
Synonymam1a
Map
Oat-2003-OglexMAM17-5_196
Oat-AxM-DH2008-240
[ Show Nearby Loci ]
Map Data
Oat, AxM Doubled Haploid, 2008
Oat-2003-OglexMAM17-5
Species
Avena sativa
Probe
AM1
Reference
ReferenceTanhuanpaa P et al. (2008) The first doubled haploid linkage map for cultivated oat. Genome 51:560-569.
Possible Orthologs
am1
[ Show all 7 ]



Probe for Locus
AM1
Locus
[ Hide all but 1 of 8 ]
AM1a
AM1b
am1
AM1
am1a
am1b
am1c
AM0001
Reference
ReferenceFu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038.
ReferenceLi CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
General Remarks
Insert contains repeat (AG)21(CAGAG)6
Polymorphic in Kanota/Ogle and Marion/Terra.
Among 20 oat cultivars, alleles at two loci were found. There were 8 alleles at locus AM1a, polymorphic information content (PIC) = 0.85, and 2 alleles at AM1b, PIC = 0.18.
In 369 Avena sterilis accessions there were 11 alleles of size 150-240 bp, PIC = 0.41.
Type
Genomic
PCR
SSR
Microsatellite
PCR primers
5'GGA TCC TCC ACG CTG TTG A
5'CTC ATC CGT ATG GGC TTT A
SSR size
204
Amplification Conditions
Average melting temperature (degrees C) = 46
Source Species
Avena sativa
Source Germplasm
Ogle (oat)